Isolation of viral RNA and DNA
Product number:
4004209
Manufacturer:
Macherey-Nagel GmbH & Co. KG
Packaging information:
Description
Properties
| Description: | NucleoSpin® 96 Tissue, Binding Plates, Proteinase K |
|---|---|
| For: | 2 x 96 preps |
| Manufacturer Article Number: | 740741.2 |
| UNSPSC: | 23-15-18-16 |
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For rapid and efficient extraction of plasmid DNA from bacterial cells.The method is based on a combination of alkaline lysis and RNase treatment to obtain a clear lysate with minimal genomic DNA and RNA contamination. The bound plasmid DNA is washed and finally eluted by adding a buffer. The test kit contains the I-Blue Lysis Buffer, an optional colour indicator. The use of this indicator prevents common handling errors that impair efficient cell lysis and neutralization.Fast extraction timeHigh yieldPrevention of handling errors during cell lysis and neutralisation by I-Blue colour indicatorSpecificationsI-Blue Mini Plasmid Kit // I-Blue Midi Plasmid KitMethod:Spin column // Anion-ExchangeSample size:1 - 7 ml // 50 - 100 ml high-copy plasmid / 100 - 150 ml low-copy plasmidBinding capacity:50 µg // 500 µgFragment size:1 - 15 kb // 1 - 20 kbTypical yield:up to 50 µg // 200 - 500 µgOperation Time:15 min. // 80 min.
The test kit was developed to obtain or concentrate DNA fragments from agarose gels, PCR or other enzymatic processes.The agarose gel is dissolved, the enzymes are denatured and the DNA fragments will bind to the glass fibre matrix in the spin column. Wash buffers (containing ethanol) are used to remove contaminants and a low salt elution buffer is used to recover the purified DNA fragments. Recoveries are 90 - 95 % for PCR clean-up. With this kit, PCR purification and gel extraction procedures can be performed, so that a second test kit is not necessary.High recovery rateShort process timeOne test kit for two methodsSpecificationsSample size:up to 300 mg of agarose gel / up to 100 µl of PCR productBinding capacity:10 µg DNAFragment size:10 kbTypical yield:80-90% gel extraction / 90-95% PCR clean-upOperation Time:20 min.
The test kit was designed specifically for purification of viral DNA/RNA from cell-free samples such as serum, plasma, body fluids, and the supernatant of viral infected cell cultures. The DNA/RNA from the viruses is lysed quickly, the enzymes are denatured and the DNA fragments will bind to the glass fibre matrix in the spin column. Contaminants such as salts, metabolites, and soluble macromolecular cellular components are removed in the wash process. The nucleic acids are eluted in RNase-free water and are then ready for use in subsequent reactions including Real-Time PCR, automated fluorescent DNA Sequencing, PCR, and other enzymatic reactions.Fast extraction timeQuality tested on a lot-to-lot basisSpecificationsMethod:Spin columnSample size:200 µlElution Volume:50 µlOperation Time:40 min.
Rapid and efficient isolation of viral DNA and RNA from serum, swabs, plasma, saliva, and other body fluids. The magnetic beads technology enables the isolation of highquality nucleic acids that are free of proteins, nucleases and other impurities. The purified nucleic acids are ready for direct use in downstream applications such as Next-Gen sequencing, Sanger Sequencing, Real-Time PCR and qPCR detection and other sensitive enzymatic testing.This kit is compatible with the most common automated systems or usable for manual procedure.High yield and high extract purityConsistent and reproducible resultsMinimal hands-on timeTemperature-stable componentsCompatible with the most common automated systemsScope of supply ( Extraction Kit ): Viral lysis buffer, magnetic beads, washing buffer 1 + 2
For rapid and efficient extraction of plasmid DNA from bacterial cells.The method is based on a combination of alkaline lysis and RNase treatment to obtain a clear lysate with minimal genomic DNA and RNA contamination. The bound plasmid DNA is washed and finally eluted by adding a buffer.SpecificationsMini Kit // Midi KitMethod:Spin column // Anion-ExchangeSample size:1 - 5 ml // 50 ml high-copy plasmid / 100 ml low-copy plasmidBinding capacity:30 µg // 800 µgFragment size:1 - 15 kb // 1 - 20 kbTypical yield:10- 30 µg // 200 - 800 µgOperation Time:15 min. // 120 min.
Spin column system consisting of filter tubes with glass fibre filter and collection tubes. For the use of leftover reagents from IBI I-Blue MINI Plasmidpurification-Kits (IB47170, IB47171, IB47172), Gel / PCR / DNA Fragment Extraction Kits (IB47010, IB47020, IB47030), Plasmidpurification-Kits High-Speed MINI (IB47101, IB47102) or similar products from other suppliers using a binding, washing and elution method. Sample size, binding capacity and elution volume listed in the specifications must be observed.SpecificationsGel Fragment Extraction Kit // Plasmidpurification-KitsSample size:300 mg agarose gel / 100 µl PCR product // 1 - 4 ml bacterial cultureBinding capacity:up to 10 µg // up to 30 µgElution volume:20 - 50 µl // 50 - 100 µl
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For rapid and efficient extraction of plasmid DNA from bacterial cells. The method is based on a combination of alkaline lysis and RNase treatment to obtain a clear lysate with minimal genomic DNA and RNA contamination. The bound plasmid DNA is washed and finally eluted by adding a buffer.Fast extraction timeHigh yieldStandard version or endotoxin-free version (<0.10 EU/µg) availableSpecificationsMethod:Anion-ExchangeSample size:200 - 800 ml high-copy plasmid / 500 - 1600 ml low-copy plasmidBinding capacity:1.5 mgFragment size:1 - 20 kbTypical yield:1 - 1.5 mgOperation time:180 min.